Mutation analysis of APC gene in gastric cancer with microsatellite instability
作者： Dian-Chun Fang
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摘要: AIM: To evaluate the role of APC mutation in gastric carcinogenesis and to correlate with microsatellite instability (MSI) carcinomas. METHODS: was measured multiplex PCR, denaturing gradient gel electrophoresis DNA sequencing; MSI analyzed by PCR-based methods. RESULTS: Sixty-eight cases sporadic carcinoma were studied for at exon 15 MSI. mutaions detected (22.1%) cancers. Frequence (33.3%) intestinal type cancer significantly higher than that diffuse (13.1%, P < 0.05). On contrary, no association observed between tumor size, differentiation, depth invasion, metastasis or clinical stages. Using five markers, least one locus 17 68 (25%) tumors analyzed. mutations all MSI-L (only locus, n = 9) MSS (tumor lacking stable, 51), but found MSI-H (≥ 2 loci, 8). CONCLUSION: is involved independent phenotype related LOH pathway cancer.
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