Forensic tracing of horse identities using urine samples and DNA markers

摘要: Abstract The possibility to carry out DNA testing using horse urine samples collected for doping control was tested nine microsatellite markers and one mitochondrial D‐loop single strand conformation polymorphism (SSCP). Genomic polymerase chain reaction (PCR) analysis prepared from different volumes of (5, 40 80 μ1 per reaction) a simple protocol. Consistent genetic data were obtained blood except in few cases where PCR the smallest volume preparation only revealed allele heterozygotes. In these serial reactions showed that two alleles randomly amplified, probably because very low number initial target molecules. most amount product increased with increasing but three drastic reduction product. For ultrafiltration concentrated dramatically improved results enabled genotyping wit...