Cellular peptide processing after a single arginyl residue. Studies on the common precursor for pancreatic polypeptide and pancreatic icosapeptide.
作者： T.W. Schwartz
DOI: 10.1016/S0021-9258(18)61158-5
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摘要: Abstract The processing of the common precursor for pancreatic polypeptide and icosapeptide was studied in primary cultures endocrine cells isolated from duodenal part canine pancreas. Biosynthetically labeled peptides were characterized by enzymatic digestion radiosequencing compared to a COOH-terminally extended form which pancreas also sequenced. It substantiated that, these cell cultures, can be at classical dibasic site between icosapeptide, monobasic its COOH-terminal extension. Pulse-chase experiments showed that cleavage occurs later than one biosynthetic process; apparently not cleaved before prohormone had been processed site. could distinguished mechanism as, time, gradually lost ability cleave while unaffected. is concluded conversion, important activation series hormones, neuropeptides, growth factors, distinct cellular mechanism.
sci-hub.st 参考文章(26)
E. Boel, T.W. Schwartz, K.E. Norris, N.P. Fiil, A cDNA encoding a small common precursor for human pancreatic polypeptide and pancreatic icosapeptide. The EMBO Journal. ,vol. 3, pp. 909- 912 ,(1984) , 10.1002/J.1460-2075.1984.TB01904.X
A B Leiter, H T Keutmann, R H Goodman, Structure of a precursor to human pancreatic polypeptide. Journal of Biological Chemistry. ,vol. 259, pp. 14702- 14705 ,(1984) , 10.1016/S0021-9258(17)42659-7
A B Leiter, M R Montminy, E Jamieson, R H Goodman, Exons of the human pancreatic polypeptide gene define functional domains of the precursor. Journal of Biological Chemistry. ,vol. 260, pp. 13013- 13017 ,(1985) , 10.1016/S0021-9258(17)38830-0
Thue W. Schwartz, The processing of peptide precursors FEBS Letters. ,vol. 200, pp. 1- 10 ,(1986) , 10.1016/0014-5793(86)80500-2
T.W. Schwartz, R.L. Gingerich, H.S. Tager, Biosynthesis of pancreatic polypeptide. Identification of a precursor and a co-synthesized product. Journal of Biological Chemistry. ,vol. 255, pp. 11494- 11498 ,(1980) , 10.1016/S0021-9258(19)70318-4
PH O'Farrell, High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry. ,vol. 250, pp. 4007- 4021 ,(1975) , 10.1016/S0021-9258(19)41496-8
B.A. Evans, R.I. Richards, Genes for the alpha and gamma subunits of mouse nerve growth factor are contiguous. The EMBO Journal. ,vol. 4, pp. 133- 138 ,(1985) , 10.1002/J.1460-2075.1985.TB02327.X
Thue W. Schwartz, Howard S. Tager, Isolation and biogenesis of a new peptide from pancreatic islets Nature. ,vol. 294, pp. 589- 591 ,(1981) , 10.1038/294589A0
Howard S. Tager, Christoph Patzelt, Richard K. Assoian, Shu Jin Chan, John R. Duguid, Donald F. Steiner, BIOSYNTHESIS OF ISLET CELL HORMONES Annals of the New York Academy of Sciences. ,vol. 343, pp. 133- 147 ,(1980) , 10.1111/J.1749-6632.1980.TB47247.X
T. Takeuchi, T. Yamada, Isolation of a cDNA clone encoding pancreatic polypeptide Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 1536- 1539 ,(1985) , 10.1073/PNAS.82.5.1536