Polymerase incorporation of a 2′-deoxynucleoside-5′-triphosphate bearing a 4-hydroxy-2-mercaptobenzimidazole nucleobase analogue
作者： Kunihiko Morihiro , Hidekazu Hoshino , Osamu Hasegawa , Yuuya Kasahara , Kohsuke Nakajima
DOI: 10.1016/J.BMCL.2015.05.075
关键词:
摘要: Abstract Here, we describe the enzymatic construction of a new larger base pair formed between adenine (A) and 4-hydroxy-2-mercaptobenzimidazole ( SB ) nucleobase analogue. We investigated incorporation 2′-deoxynucleoside-5′-triphosphate bearing analogue dSBTP into oligonucleotides (ONs) by DNA polymerases. could be effectively incorporated at site opposite dA in template several B family These findings provide insights various aspects biotechnology, including design non-natural pairs.
elsevier.com
sci-hub.se 参考文章(37)
Marcel Hollenstein, Deoxynucleoside triphosphates bearing histamine, carboxylic acid, and hydroxyl residues--synthesis and biochemical characterization. Organic and Biomolecular Chemistry. ,vol. 11, pp. 5162- 5172 ,(2013) , 10.1039/C3OB40842F
Katrin Groebke, J�rg Hunziker, William Fraser, Ling Peng, Ulf Diederichsen, Kaspar Zimmermann, Armin Holzner, Christian Leumann, Albert Eschenmoser, Warum Pentose‐ und nicht Hexose‐Nucleinsäuren??. Teil V. (Purin‐Purin)‐Basenpaarung in der homo‐DNS‐Reihe: Guanin, Isoguanin, 2,6‐Diaminopurin und Xanthin Helvetica Chimica Acta. ,vol. 81, pp. 375- 474 ,(1998) , 10.1002/HLCA.19980810302
Stefan Jäger, Goran Rasched, Hagit Kornreich-Leshem, Marianne Engeser, Oliver Thum, Michael Famulok, A Versatile Toolbox for Variable DNA Functionalization at High Density Journal of the American Chemical Society. ,vol. 127, pp. 15071- 15082 ,(2005) , 10.1021/JA051725B
Michael Strerath, Andreas Marx, Tuning PCR Specificity by Chemically Modified Primer Probes Angewandte Chemie International Edition. ,vol. 41, pp. 4766- 4769 ,(2002) , 10.1002/ANIE.200290043
Francesca Di Pasquale, Daniela Fischer, Dina Grohmann, Tobias Restle, Armin Geyer, Andreas Marx, Opposed Steric Constraints in Human DNA Polymerase β and E. coli DNA Polymerase I Journal of the American Chemical Society. ,vol. 130, pp. 10748- 10757 ,(2008) , 10.1021/JA8028284
Ken-ichi Miyata, Ryuji Tamamushi, Hirosuke Tsunoda, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine, Synthesis and triplex formation of oligonucleotides containing 8-thioxodeoxyadenosine. Organic Letters. ,vol. 11, pp. 605- 608 ,(2009) , 10.1021/OL802622S
Michiko Kimoto, Rie Yamashige, Ken-ichiro Matsunaga, Shigeyuki Yokoyama, Ichiro Hirao, Generation of high-affinity DNA aptamers using an expanded genetic alphabet. Nature Biotechnology. ,vol. 31, pp. 453- 457 ,(2013) , 10.1038/NBT.2556
Angie M. Kretulskie, Thomas E. Spratt, Structure of purine-purine mispairs during misincorporation and extension by Escherichia coli DNA polymerase I. Biochemistry. ,vol. 45, pp. 3740- 3746 ,(2006) , 10.1021/BI052306U
Yunfeng Cheng, Hanjing Peng, Weixuan Chen, Nanting Ni, Bowen Ke, Chaofeng Dai, Binghe Wang, Rapid and specific post-synthesis modification of DNA through a biocompatible condensation of 1,2-aminothiols with 2-cyanobenzothiazole. Chemistry: A European Journal. ,vol. 19, pp. 4036- 4042 ,(2013) , 10.1002/CHEM.201201677
Michelle L. Hamm, Anna J. Parker, Tyler W. E. Steele, Jennifer L. Carman, Carol A. Parish, Oligonucleotide incorporation and base pair stability of 9-deaza-2'-deoxyguanosine, an analogue of 8-oxo-2'-deoxyguanosine. Journal of Organic Chemistry. ,vol. 75, pp. 5661- 5669 ,(2010) , 10.1021/JO1010763