Blue Light Rescues Mice from Potentially Fatal Pseudomonas aeruginosa Burn Infection: Efficacy, Safety, and Mechanism of Action

摘要: Blue light has attracted increasing attention due to its intrinsic antimicrobial effect without the addition of exogenous photosensitizers. However, use blue for wound infections not been established yet. In this study, we demonstrated efficacy at 415 nm treatment acute, potentially lethal Pseudomonas aeruginosa burn in mice. Our vitro studies that inactivation rate P. cells by was approximately 35-fold higher than keratinocytes (P = 0.0014). Transmission electron microscopy revealed light-mediated intracellular damage cells. Fluorescence spectroscopy suggested coproporphyrin III and/or uroporphyrin are possibly photosensitive chromophores associated with aeruginosa. vivo using an bioluminescence imaging technique and area-under-the-bioluminescence-time-curve (AUBC) analysis showed a single exposure 55.8 J/cm2, applied 30 min after bacterial inoculation infected mouse burns, reduced AUBC 100-fold comparison untreated burns < 0.0001). Histological analyses terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) assays indicated no significant skin exposed effective dose. Survival increased survival mice from 18.2% 100% conclusion, therapy might offer safe alternative conventional infections.