In vitro mineral nutrition of Curcuma longa L. affects production of volatile compounds in rhizomes after transfer to the greenhouse

摘要: Turmeric is a rich source of bioactive compounds useful in both medicine and cuisine. Mineral concentrations effects (PO43−, Ca2+, Mg2+, KNO3) were tested during vitro rhizome development on the ex content volatile constituents rhizomes after 6 months greenhouse. A response surface method (D-optimal criteria) was repeated high low-input fertilizer treatments. Control plants grown Murashige Skoog (MS) medium, acclimatized greenhouse field. The investigated by GC-MS. total volatiles affected treatments, treatment with Ca2+ KNO3; but PO43− Mg2+ had no significant effect. higher high-input treatments (49.7 ± 9 mg/g DM) 4 mM 60 mM KNO3 5 mM NH4+, than (26.6 ± 9 mg/g DM), MS control (15.28 ± 2.7 mg/g DM; 3 mM 20 mM K+, 39 mM NO3−, 20 mM 1.25 mM PO43−, 1.5 mM Mg2+). interaction curcumenol isomer I II, germacrone, isocurcumenol, β-elemenone content. Increasing phosphate concentration to 6.25 mM increased neocurdione methenolone contents. These results show that minerals bioreactor medium biosynthesis turmeric components transfer six months later. multi-factor design identified 1) nutrient regulation specific within unique phytochemical profile for Curcuma longa L. clone 35–1 2) varied profiles maintained integrity growth fertility conditions.