Evidence for the involvement of TNF-α, IL-1β and IL-10 in the antinociceptive and anti-inflammatory effects of indole-3-guanylhydrazone hydrochloride, an aromatic aminoguanidine, in rodents.

摘要: Abstract Background Indole-3-guanylhydrazone hydrochloride (LQM01) is a new derivative of aminoguanidine hydrochloride, an aromatic aminoguanidine. Methods Mice were treated with LQM01 (5, 10, 25 or 50 mg/kg, i.p.), vehicle (0.9% saline i.p.) standard drug. The mice subjected to carrageenan-induced pleurisy, abdominal writhing induced by acetic acid, the formalin test and hot-plate test. model non-inflammatory chronic muscle pain acid was also used. from protocol assessed for withdrawal threshold, strength motor coordination. evaluated Fos protein. Results inhibits TNF-α IL-1β production, as well leukocyte recruitment during inflammation process. level IL-10 in LQM01-treated increased pleural fluid. In addition, decreased nociceptive behavior test, (both phases) latency time on hot-plate. treatment mechanical hyperalgesia pain, no changes reduced number positive cells superficial dorsal horn. This compound exhibited antioxidant properties vitro assays. Conclusions has outstanding anti-inflammatory analgesic profile, probably mediated through reduction proinflammatory cytokines release, increase production neuron activity horn spinal cord mice. General significance Beneficial effects suggest that it some important clinical features can play role management ‘dysfunctional pain’ inflammatory diseases.