Dry mass and area changes in the GM-10 skin fibroblasts during aging in vitro
作者： Paraskevi M. BeMiller , John E. Miller
DOI: 10.1007/BF02431733
关键词:
摘要: Normal, human diploid skin fibroblasts (GM-10) were grown in culture and the changes mean cellular, nuclear nucleolar dry mass area monitored simultaneously at regular intervals until last passage. The results which analyzed statistically showed that all measures increased during aging vitro with a most striking surge for values from passage 29 to 31. of cell, nucleus nucleosis well correlated same structures. are discussed possible reasons findings offered.
springer.com
sci-hub.se 参考文章(17)
M KLASS, J SMITHSONNEBORN, Studies on DNA content, RNA synthesis, and DNA template activity in aging cells of Paramecium aurelia Experimental Cell Research. ,vol. 98, pp. 63- 72 ,(1976) , 10.1016/0014-4827(76)90463-8
P.D. Bowman, R.L. Meek, C.W. Daniel, Aging of human fibroblasts in vitro: Correlations between DNA synthetic ability and cell size Experimental Cell Research. ,vol. 93, pp. 184- 190 ,(1975) , 10.1016/0014-4827(75)90438-3
V.J. Cristofalo, Barbara B. Sharf, Cellular senescence and DNA synthesis. Thymidine incorporation as a measure of population age in human diploid cells. Experimental Cell Research. ,vol. 76, pp. 419- 427 ,(1973) , 10.1016/0014-4827(73)90394-7
E. H. McConkey, J. W. Hopkins, THE RELATIONSHIP OF THE NUCLEOLUS TO THE SYNTHESIS OF RIBOSOMAL RNA IN HELA CELLS Proceedings of the National Academy of Sciences of the United States of America. ,vol. 51, pp. 1197- 1204 ,(1964) , 10.1073/PNAS.51.6.1197
A. Macieira-Coelho, J. Pontén, L. Philipson, The division cycle and RNA-synthesis in diploid human cells at different passage levels in vitro. Experimental Cell Research. ,vol. 42, pp. 673- 684 ,(1966) , 10.1016/0014-4827(66)90280-1
R. HOLLIDAY, G. M. TARRANT, Altered enzymes in ageing human fibroblasts. Nature. ,vol. 238, pp. 26- 30 ,(1972) , 10.1038/238026A0
V.J Cristofalo, D. Kritchevsky, Cell size and nucleic acid content in the diploid human cell line WI-38 during aging. Pharmacology. ,vol. 19, pp. 313- 320 ,(1969) , 10.1159/000137216
J.R. Nilsson, V. Leick, Nucleolar organization and ribosome formation in Tetrahymena pyriformis GL Experimental Cell Research. ,vol. 60, pp. 361- 372 ,(1970) , 10.1016/0014-4827(70)90529-X
Paraskevi M. Bemiller, Lieh-Hauh Lee, Nucleolar changes in senescing WI-38 cells. Mechanisms of Ageing and Development. ,vol. 8, pp. 417- 427 ,(1978) , 10.1016/0047-6374(78)90041-6
Ivan L. Cameron, E. Ernest Guile, NUCLEOLAR AND BIOCHEMICAL CHANGES DURING UNBALANCED GROWTH OF TETRAHYMENA PYRIFORMIS Journal of Cell Biology. ,vol. 26, pp. 845- 855 ,(1965) , 10.1083/JCB.26.3.845