Biphasic effects of prostaglandin E2 on bone formation in cultured fetal rat calvariae: interaction with cortisol.

摘要: Previous studies have shown that prostaglandin E2 (PGE2) has both inhibitory and stimulatory effects on the incorporation of proline into collagenase-digestible protein (CDP) in cultured fetal rat calvaria. The present were undertaken to analyze further these biphasic PGE2. PGE2 increased [3H]thymidine at 24 h, this effect was enhanced presence cortisol (10-8 10-7M). An CDP labeling observed 96 h with (10-6 M) absence or indomethacin M), but not 10-7 M). When central osteoblast-rich bone periosteum analyzed separately, PGE2, without indomethacin, confined bone. Addition aphidicolin (30 μM), an inhibitor cell replication, did prevent labeling. Analysis labeled collagen by polyacrylamide gel electrophoresis showed a decrease type I coll...