Molecular detection and characterization of sapovirus in hospitalized children with acute gastroenteritis in the Philippines

摘要: Abstract Background Human sapovirus (SaV) is a causative agent of acute gastroenteritis. Recently, SaV detection has been increasing worldwide due to the emerging genotype I.2. However, infection not reported in Philippines. Objectives To evaluate prevalence and genetic diversity hospitalized children aged less than 5 years with Study design Stool samples were collected from gastroenteritis at three hospitals Philippines June 2012 August 2013. was detected by reverse transcription real-time PCR, polymerase capsid gene sequences analyzed. Full genome sequencing recombination analysis performed on possible recombinant viruses. Results 7.0% tested stool (29/417). In 10 SaV-positive cases, other viruses also detected, including rotavirus ( n =6), norovirus =2), human astrovirus =2). Four known genotypes (GI.1 [7], GI.2 [2], GII.1 [12], GV [2]) one novel =3) identified sequence analysis. revealed that 5ʹ nontranslated region (NTR) nonstructural protein closely related Bristol/98/UK variant, whereas structural 3ʹ NTR GII.4 Kumamoto6/Mar2003/JPN variant. Discussion conclusions regularly during study period. A recombinant, GII.1/GII.4, cases two different sites.