Synthesis of a stable analog of the phosphorylated form of CheY: phosphono-CheY.
作者： Christopher J. Halkides , Cory J. Bottone , Eric S. Casper , R. Matthew Haas , Kenneth McAdams
DOI: 10.1016/S0076-6879(06)22016-0
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摘要: Abstract The chemical modification of a cysteinyl residue D57C CheY by the addition phosphonomethyl group, (HO) 2 P(O)‐CH ‐, is described. This produces nonlabile analog an aspartyl phosphate in active form CheY. chemically modified protein, phosphono‐CheY, suitable for structural and functional studies. An extensive discussion synthetic methodology purification strategy presented. A detailed protocol given.
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