A common requirement for p21c-ras function in the mitogenic signaling pathways of Swiss 3T3 fibroblasts.

摘要: The role of p21c-ras in the activation DNA synthesis quiescent Swiss 3T3 fibroblasts was examined by scrape loading or microinjecting neutralizing monoclonal antibody Y13-259 into cells. delayed but did not block both serum-stimulated entry cells S phase and accumulation cdc2 mRNA protein at G1-S boundary. Introduction also stimulated expression sufficient to neutralize loaded antibody; this finding suggests that delay is attributable time taken synthesize an excess over implies autoregulation c-ras expression. had no effect upon phosphoinositide-mediated responses ([Ca2+]i increase kinase C) platelet-derived growth factor bombesin, demonstrating required for mitogen C. inhibited 5-bromo-2'-deoxyuridine incorporation response all combinations mitogens used stimulate (fetal calf serum, factor, combination insulin with 12-O-tetradecanoylphorbol-13-acetate, epidermal prostaglandin E1), indicating functions on pathways common effective examined.