Chapter 12 Isolation and Fractionation of Metaphase Chromosomes
作者： Norman P. Salzman , John Mendelsohn
DOI: 10.1016/S0091-679X(08)62123-8
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摘要: Publisher Summary Animal cells maintained in cell culture the logarithmic phase of growth provide a ready source rapidly dividing cells. By addition mitotic inhibitor to these cultures, it is possible obtain many metaphase arrest. This chapter describes procedures that have been used laboratory isolate chromosomes from cultures.. The procedure has distinctive size groups Chinese hamster also described. These studies were carried out with suspension culture. method propagation facilitates accumulation large numbers fractionated are currently available should prove valuable as three distinct genetic classes DNA. It hoped assignment gene loci chromosome can be made using hybridization techniques. Vinblastine sulfate arrest at metaphase. illustrates percentage HeLa accumulated different times after vinblastine.
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