Engineering of a synthetic electron conduit in living cells
作者： H. M. Jensen , A. E. Albers , K. R. Malley , Y. Y. Londer , B. E. Cohen
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摘要: Engineering efficient, directional electronic communication between living and nonliving systems has the potential to combine unique characteristics of both materials for advanced biotechnological applications. However, cell membrane is designed by nature be an insulator, restricting flow charged species; therefore, introducing a biocompatible pathway transferring electrons across without disrupting significant challenge. Here we describe genetic strategy move intracellular inorganic extracellular acceptor along molecularly defined route. To do so, reconstitute portion electron transfer chain Shewanella oneidensis MR-1 into model microbe Escherichia coli. This engineered E. coli can reduce metal ions solid oxides ∼8× ∼4× faster than its parental strain. We also find that oxide reduction more efficient when nanoscale dimensions. work demonstrates cassette create conduit from cells materials, it highlights importance matching size scale protein donors acceptors.
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