Actin in Mung Bean Mitochondria and Implications for Its Function

摘要: Here, a large fraction of plant mitochondrial actin was found to be resistant protease and high-salt treatments, suggesting it protected by membranes. A portion this became sensitive or treatment after removal the outer membrane, indicating that some is located inside membrane. The import an actin–green fluorescent protein (GFP) fusion into mitochondria in transgenic plant, actin:GFP, visualized living cells demonstrated flow cytometry immunoblot analyses. Polymerized actin:GFP plants mung bean (Vigna radiata). Notably, associated with purified from early-developing cotyledons during seed germination treatments. With cotyledon ageing, more both progressive appeared occur concert conversion quiescent active forms germination. binding DNA (mtDNA) liquid chromatography–tandem mass spectrometry analysis. Porin ADP/ATP carrier proteins were also mtDNA-protein complexes. Treatment depolymerization reagent reduced membrane potential triggered release cytochrome C. function possible pathway are discussed.