The Cebpa +37‐kb enhancer directs transgene expression to myeloid progenitors and to long‐term hematopoietic stem cells
作者： Hong Guo , Ou Ma , Alan D. Friedman
关键词:
摘要: C/EBPα is expressed preferentially in myeloid compared with lymphoid or erythroid cells and directs lineage specification. also at lower levels HSCs several nonhematopoietic tissues. The Cebpa gene has a conserved, 450-bp segment +37 kb that harbors enhancer-specific epigenetic marks activate cell line. Herein, we characterize transgenic C57BL/6 mice, which the enhancer 845-bp promoter regulate hCD4 reporter. FACS analysis, vitro colony assays, vivo competitive secondary transplantation revealed but not MEPs progenitors functional LT-HSCs are found almost exclusively Cebpa-hCD4+ hCD4− marrow population. hCD4+ CMP yielded predominantly myeloid, whereas generated mainly Meg/E colonies. Providing insight into control of maturation, Pu.1 RNAs were CMP, Scl, Gata2, Gata1, Klf1, Ets1, Fli1 predominated Runx1, Myb, HoxA9, Erg similar both. Cebpa-hCD4 transgene expression was lacking multiple In summary, +37-kb sufficient for progenitor LT-HSC-specific expression.
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