Amniotic membrane‐mesenchymal stromal cells secreted factors and extracellular vesicle‐miRNAs: Anti‐inflammatory and regenerative features for musculoskeletal tissues

摘要: Human amniotic membrane-derived mesenchymal stromal cells (hAMSCs) are easily obtained in large quantities and free from ethical concerns. Promising therapeutic results for both hAMSCs their secreted factors (secretome) were described by several vitro preclinical studies, often treatment of orthopedic disorders such as osteoarthritis (OA) tendinopathy. For clinical translation the hAMSC secretome cell-free therapy, a detailed characterization hAMSC-secreted is mandatory. Herein, we tested presence 200 754 miRNAs extracellular vesicles (EVs). Thirty-seven cytokines/chemokines identified at varying abundance, some which involved chemotaxis homeostasis inflammatory positive remodeling matrix, damaged tendinopathy OA. We also found 336 EV-miRNAs, 51 accounted more than 95% genetic message. A focused analysis based on related to OA showed that most abundant EV-miRNAs teno- chondro-protective, able induce M2 macrophage polarization, inhibit T cells, promote Treg. Functional IL-1β treated tenocytes chondrocytes resulted downregulation inflammation-associated genes. Overall, key regulatory molecules explain promising musculoskeletal conditions groundwork similar studies other pathologies. Furthermore, will pave way future aimed sharply predicting disease-targeted efficacy, well setting up potency release assays fingerprint clinical-grade batches whole or purified components.