Differentiation of the O-2A progenitor cell line CG-4 into oligodendrocytes and astrocytes following transplantation into glia-deficient areas of CNS white matter.

摘要: The in vitro properties of the CG4 cell line have led to its increasing use as a with which study behaviour O-2A progenitor cell. In this we examined vivo following transplantation into areas adult rat spinal cord white matter been permanently depleted glial cells by combination local X-irradiation and direct injection 0.1% ethidium bromide. Twenty-one days after transplantation, both myelin-forming oligodendrocytes fibrillary acidic protein-positive astrocytes were identified within lesion, indicating that has bipotential differentiation when introduced pathological environment consisting demyelinated axons but devoid or astrocytes. respect, resembles other lines transplanted similar lesions. some remyelination was observed extent achieved growth factor-expanded populations cells. transplant origin types lesion confirmed retroviral incorporation lacZ marker gene, expression allowed their identification histochemistry. conclusion, make it highly suitable progenitors normal damaged CNS.