Dynamics and functions of bacterial communities in bark, charcoal and sand filters treating greywater

摘要: Abstract This study explored the effects of greywater application on dynamics and functions biofilms developed in bark, activated charcoal sand filters used for removal organic matter nitrogen. Duplicate columns (20 cm diameter, 60 cm deep) were packed with or effective size 1.4 mm uniformity coefficient 2.2, dosed 32 L m−2 day−1 an artificial (14 g BOD5 m−2 day−1) 116 days. Potential respiration rate (PRR), determined filter samples after addition excess glucose, bacterial diversity composition, analysed by 454-pyrosequencing 16S ribosomal DNA, measured at different times depths filters. The bark more efficient removing BOD5 than (98, 97% 75%, respectively). highest PRR 0–2 cm layer day 84 was found filters, followed (632 ± 66, 222 ± 34 56 ± 2 mg O2 L−1, respectively; n = 2). Bacterial community showed richness. both diverse dynamic (changing over time depth) communities bark. In to greywater, lignocelluosic composition its lower pH probably selected structure content provided additional substrate, as shown higher nitrifying genera. oligotrophic sand, itself defined community. Thus, initially low biomass latter enriched time, allowing a diversified develop. top layers displayed high dominance Rhizobium, Pseudomonas Acinetobacter, which less evident layer, whereas these genera prominent 60 cm. PRR, profiles indicated that degradation occurred mainly 20 cm means could be designed shallower