The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase.
作者： Manuela Zaccolo , Ermanno Gherardi
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摘要: … The purpose of this study was to address the effect of mutational load in protein evolution experiments in vitro. As a model system, we have evolved the bacterial enzyme TEM-1 for the …
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