Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration.

摘要: The rapid and reliable detection of infectious agents is one the most challenging tasks in scenarios lacking well-equipped laboratory infrastructure, like diagnostics rural areas developing countries. Commercially available point-of-care diagnostic tests for emerging rare diseases are particularly scarce. In this work we present a test Orthopoxviruses (OPV). OPV ABICAP assay detects down to 1 × 104 plaque forming units/mL particles within 45 min. It can be applied clinical material skin crusts all zoonotic infecting humans, including Vaccinia, Cowpox, Monkeypox, importantly Variola virus. Given high sensitivity ease handling, novel could highly useful on-site suspected Monkeypox virus infections proper infrastructure as well testing bioterrorism samples.