Conotoxin GI: disulfide bridges, synthesis, and preparation of iodinated derivatives.
作者： WR Gray , TA Luque , R Galyean , E Atherton , RC Sheppard
DOI: 10.1021/BI00307A040
关键词:
摘要: The 13 amino acid toxic peptide from the marine snail Conus geographus, conotoxin GI, blocks acetylcholine receptor at neuromuscular junction. In this report, we describe a method for analyzing disulfide bonding in nanomole amounts of small cystine-rich peptides. procedure involves partial reduction and double-label alkylation cysteine residues. Using method, show that natural GI has (2-7, 3-13) configuration. structure been confirmed by chemical synthesis. preparation purification molecularly homogeneous, iodinated derivatives toxin are also described. All derivatives, including [diiodohistidine,diiodotyrosine]conotoxin retained least half biological activity unmodified toxin. Since tetraiodinated toxin, which is greater than 25% weight iodine, retains considerable toxicity, histidine tyrosine residues not crucial activity.
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