Overexpression of cytochrome P450 genes in pyrethroid-resistant Culex quinquefasciatus
作者： Osamu Komagata , Shinji Kasai , Takashi Tomita
DOI: 10.1016/J.IBMB.2010.01.006
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摘要: JPal-per strain of Culex quinquefasciatus exhibits extremely high resistance against pyrethroids in larvae, though the is greatly lower adults. Increased microsome monooxygenase metabolism one major factors larval this strain. We cloned 46 novel cytochrome P450 cDNAs from An oligonucleotide microarray was designed for genes plus 16 previously reported along with other non-P450 gene probes. Of these, five were upregulated (>2.5-fold) larvae as compared a susceptible The expression ratios highest three among screened analysis, CYP9M10, CYP4H34 and CYP6Z10, further validated by qPCR 264-, 8.3-, 3.9-fold, respectively. In JPal-per, transcription levels CYP9M10 showed similar stage-dependent pattern level during larvfrom Ogasawara Islands Japanal stage dramatically decreases adult stage. This specific overexpression manner two consistent characteristic reported, suggesting that P450s, CYP4H34, are involved pyrethroid detoxification
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