Isolation and characterization of multiple cell types from a single human colonic carcinoma: tumourigenicity of these cell types in a xenograft system.

摘要: The HCA-7 cell line was established from a moderately well differentiated mucinous adenocarcinoma of the colon, which showed histological heterogeneity. This reflected in morphological heterogeneity early passages but diminished as cells were passaged vitro. Nine subpopulations isolated passage cultures and maintained lines. Each subpopulation demonstrated unique set stable biological characteristics When vivo, there wide variation xenograft generation time. parent gave rise to six distinct patterns, two had been observed primary carcinoma. Individual yielded characteristic tumours composed between one four organizational patterns. Xenografts differed both organization differentiation within different Mucous absent some while abundant others. provide new extensive model system for studying maintenance phenotypic colorectal