Involvement of TRPC Channels in Lung Cancer Cell Differentiation and the Correlation Analysis in Human Non-Small Cell Lung Cancer

摘要: The canonical transient receptor potential (TRPC) channels are Ca(2+)-permeable cationic controlling the Ca(2+) influx evoked by G protein-coupled activation and/or store depletion. Here we investigate involvement of TRPCs in cell differentiation lung cancer. expression and correlation to cancer grade non-small (NSCLC) were analyzed real-time PCR immunostaining using tissue microarrays from 28 patient samples. association with was also investigated line A549 Western blotting. channel activity monitored imaging patch recording after treatment all-trans-retinoic acid (ATRA). TRPC1, 3, 4 6 correlated NSCLC patients, but there no age, sex, smoking history type. ATRA upregulated TRPC3, TRPC4 TRPC6 enhanced cells, however, showed direct effect on TRPC channels. Inhibition pore-blocking antibodies decreased mitosis, which counteracted chronic ATRA. Blockade inhibited proliferation, while overexpression increased proliferation. We conclude that correlates differentiation. mediate pharmacological play important roles regulating gives a new understanding biology anti-cancer therapy.