Vascular endothelial growth factor production is induced by histone deacetylase 1 and suppressed by von Hippel-Lindau protein in HaCaT cells.

摘要: Purpose: In hypoxic tumoral tissues, vascular endothelial growth factor (VEGF) expression is positively regulated by histone deacetylase 1 (HDAC1) and negatively the tumour suppressor protein von Hippel-Lindau (VHL) via transforming factor-alpha (HIF-1alpha). It has been reported that VEGF, HDAC1 LL-37, but not VHL, are over-expressed in psoriatic skin. Although HIF-1alpha constitutively expressed normal keratinocytes, it known if VHL can regulate VEGF production these cells. Methods: The participation of regulation HDAC-, VHL- LL-37-transfected HaCaT cells, cells treated with inhibitors, was studied. Results: increased HDAC1- maintained VHL-transfected under conditions; meanwhile, decreased TSA, transfected HDAC1-siRNA, co-transfected HIF-1alpha-siRNA pHDAC-1 levels cytoplasmic were high pLL37-transfected low pVHL- pHDAC1-transfected cells; however, detected nucleus HDAC1-transfected pHDAC1- pLL-37, when pVHL present. Conclusions: These data demonstrate HDAC1, LL-37 modulate