Gallic acid-coated sliver nanoparticle alters the expression of radiation-induced epithelial-mesenchymal transition in non-small lung cancer cells.

摘要: Abstract Background Radiotherapy is the most widely used treatment method for treating cancer with or without surgery and chemotherapy. In lung cancer, it one of important steps in excising tumor from tissue; unfortunately, radiation can induce epithelial- mesenchymal transition (EMT), a typical physiological process which cuboidal shaped epithelial cell loses its phenotype acquires mesenchymal-like thus, increases metastasis progression body. To prevent EMT mediated metastasis, we aimed to 1) synthesize silver nanoparticles by using Gallic acid, potential antioxidant acts as stabilizing reducing agent form nanoparticle (GA-AgNPs) 2) analyze effect on markers during radiation-induced A549 cells. Methods cells were irradiated 8Gy (X-ray) treated GA-AgNPs at fixed concentration under vitro condition. prepared characterized absorption, stability, size morphology UV–Visible spectrophotometer, Zeta Transmission electron microscopy respectively. After irradiation, changes observed an inverted microscope, gene protein expression analyzed RT-PCR western blotting. Results/conclusion are nano fair stability. The synthesized suppressed including Vimentin, N-cadherin, Snail-1 increased E-cadherin might inhibit acquire radio resistant potential.