Partial characterization of viral DNA from human genital warts (condylomata acuminata)

摘要: By centrifuging total cellular DNA derived from human genital warts (condylomata acuminata) in CsCl-ethidium bromide gradients, supercoiled was isolated. The molecular weight of this determined by agarose gel electrophoresis and amounted to 5.1 X 10(4). This isolated an individual wart annealed fractions aqueous supernatants the same after prior centrifugation material CsCl density gradients. Annealing observed at a approximately 1.32 g/ml corresponding expected papilloma virus particles. Since such particles were also preparation electron microscopy, it concluded that molecules nucleocapsids. Positive hybridization found with six additional preparations warts. Therefore, seems prevails condylomata acuminata. is different other five types viruses described thus far regard its restriction endonuclease cleavage patterns. analyzed tentatively designated as type 6 (HPV 6).