Effect of soybean ureases on seed germination and plant development.

摘要: Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The (nitrogen (N) product urease activity) is incorporated into organic compounds. Thus, involved in N remobilization, as well primary assimilation. Two isoforms have been described for soybean: embryo-specific, encoded by Eu1 gene, ubiquitous urease, Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes putative protein SBU-III. present study aimed evaluate contribution soybean ureases seed germination plant development. Analyses were performed using Eu1/Eu4/Eu5-co-suppressed transgenic plants mutants Eu4 structural genes, a urease-null mutant (eu3-a) that activates neither nor embryo-specific ureases. co-suppressed presented developmental delay during first month after germination; shoots roots significantly smaller lighter. Slower development observed double eu1-a/eu4-a eu3-a single mutant. content lower than non-transgenic plants. Among mutants, lowest eu1-a highest content. Altogether, these results indicate increased ureolytic activity plays an important role