Nonenzymatic separation of myocardial cell nuclei from whole heart tissue.
作者： C. C. Liew , G. Jackowski , T. Ma , M. J. Sole
DOI: 10.1152/AJPCELL.1983.244.1.C3
关键词:
摘要: A simple and rapid nonenzymatic method has been developed to isolate myocardial cell nuclei from whole heart tissue. This consists of a controlled disruption cells followed by isopycnic gradient centrifugation. We have reviewed compared our others more lengthy laborious. By using number criteria, such as morphometric measurements, chemical composition, functional studies, specific nuclear protein markers, mathematical analysis, we show that the digestion provides most useful technique for study biochemistry nucleus.
sci-hub.se 参考文章(32)
J. Austoker, D. Cox, A. P. Mathias, Fractionation of nuclei from brain by zonal centrifugation and a study of the ribonucleic acid polymerase activity in the various classes of nuclei. Biochemical Journal. ,vol. 129, pp. 1139- 1155 ,(1972) , 10.1042/BJ1291139
M E Haines, I R Johnston, A P Mathias, D. Ridge, The synthesis of nicotinamide–adenine dinucleotide and poly(adenosine diphosphate ribose) in various classes of rat liver nuclei Biochemical Journal. ,vol. 115, pp. 881- 887 ,(1969) , 10.1042/BJ1150881
G Jackowski, C C Liew, Fractionation of rat ventricular nuclei Biochemical Journal. ,vol. 188, pp. 363- 373 ,(1980) , 10.1042/BJ1880363
Melvin R. Glick, Alastair H. Burns, William J. Reddy, Dispersion and isolation of beating cells from adult rat heart Analytical Biochemistry. ,vol. 61, pp. 32- 42 ,(1974) , 10.1016/0003-2697(74)90329-7
S. Bloom, Spontaneous rhythmic contraction of separated heart muscle cells. Science. ,vol. 167, pp. 1727- 1729 ,(1970) , 10.1126/SCIENCE.167.3926.1727
Race L. Kao, Earle W. Christman, Sinnam L. Luh, Jane M. Krauhs, G.Frank O. Tyers, Edward H. Williams, The effects of insulin and anoxia on the metabolism of isolated mature rat cardiac myocytes. Archives of Biochemistry and Biophysics. ,vol. 203, pp. 587- 599 ,(1980) , 10.1016/0003-9861(80)90216-7
M Clark, An improved procedure for the high-yield preparation of intact beating heart cells from the adult rat biochemical and morphologic study Journal of Molecular and Cellular Cardiology. ,vol. 10, pp. 1101- 1114 ,(1978) , 10.1016/0022-2828(78)90355-3
C LIEW, N MAN, G MORRIS, Histone acetylation in differentiating chick myoblast cultures Cell Biology International Reports. ,vol. 4, pp. 711- 716 ,(1980) , 10.1016/0309-1651(80)90212-X
I. Harary, B. Farley, In vitro Studies of Single Isolated Beating Heart Cells Science. ,vol. 131, pp. 1674- 1675 ,(1960) , 10.1126/SCIENCE.131.3414.1674
A.P. Mathias, Coral V.A. Wynter, The uses of metrizamide in the fractionation of nuclei from brain and liver tissue by zonal centrifugation FEBS Letters. ,vol. 33, pp. 18- 22 ,(1973) , 10.1016/0014-5793(73)80149-8