Cytotoxicity of human pI 7 interleukin-1 for pancreatic islets of Langerhans.
作者： K Bendtzen , T Mandrup-Poulsen , J Nerup , J. Nielsen , C. Dinarello
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摘要: Activated mononuclear cells appear to be important effector in autoimmune beta cell destruction leading insulin-dependent (type 1) diabetes mellitus. Conditioned medium from activated (from human blood) is cytotoxic isolated rat and islets of Langerhans. This activity was eliminated crude cytokine preparations by adsorption with immobilized, purified antibody interleukin-1 (IL-1). The islet-inhibitory the IL-1 (determined its comitogenic effect on thymocytes) were recovered acid wash. Purified natural recombinant derived predominant pI 7 form IL-1, consistently inhibited insulin response. 6 5 forms ineffective. Natural exhibited similar dose responses their thymocyte-stimulatory activity. Concentrations that islet picomolar range. Hence, monocyte-derived may contribute damage therefore development
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