Expression of γ-glutamylcysteine synthetase (γ-GCS) and multidrug resistance-associated protein (MRP), but not human canalicular multispecific organic anion transporter (cMOAT), genes correlates with exposure of human lung cancers to platinum drugs

摘要: We examined the steady-state levels of mRNA for gamma-glutamylcysteine synthetase (gamma-GCS), multidrug resistance-associated protein (MRP) and human canalicular multispecific organic anion transporter (cMOAT) in lung cancer specimens to elucidate their roles relation platinum drug resistance vivo. Seventy-six autopsy samples (38 primary tumours corresponding normal tissues) obtained from 38 patients were analysed using quantitative reverse transcription polymerase chain reaction (RT-PCR) method. Both subunits (heavy light subunits) gamma-GCS expression tumour tissues exposed drugs during life significantly higher than those non-exposed tissues, whereas only MRP elevated association with ante-mortem exposure. The correlated significantly. cMOAT did not correlate Next, we monitored heavy subunit peripheral mononuclear cells eight previously untreated after administration, which revealed that these induced These results suggest is by vivo and/or physiological stress response xenobiotics.