GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells

摘要: Background Nowadays, smart synthesized nanostructures have attracted wide attention in the field of stem cell nanotechnology due to their effect on different properties cells. Methods GFc7 growth nanofactor was based nanochelating technology as an iron-containing copper chelator nanocomplex. The this nanocomplex expansion and differentiation hematopoietic cells (HSCs) well its performance a cryoprotectant evaluated present study. Results results showed that absolute count CD34+ CD34+CD38- days 4, 7, 10 13; percentage lactate dehydrogenase enzyme same CD34+CXCR4 population day were significantly increased when they treated with fetal bovine serum (FBS)-free medium. This medium also led delayed HSCs. One noticeable result cultured FBS immediately differentiated into CD34+CD38+ cells, while did not show such immediate significant differentiation. De-freezing GFc7-treated which already frozen according cord blood bank protocols, higher viability larger number colonies colony-forming assay compared control. Conclusion It can be claimed treating HSCs leads quality quantity improvement HSCs, both terms vitro freezing de-freezing processes.