Quantitation of acidic capsular polysaccharides by Alcian blue binding.

摘要: Alcian blue is a cationic dye used to stain acidic polysaccharides, including bacterial capsules, and as biochemical reagent quantitate glycosaminoglycans glycoproteins. capsular polysaccharide (CP) complex formation can be quantitated by measuring the absorption at 620 nm of released from in 40% manoxol-1B. Optimal formation, which dependent on pH buffer well MgCl2 concentration, determined for purified CPs Neisseria meningitidis group A, Hemophilus influenzae type b, Streptococcus pneumoniae III. Under optimal electrolyte conditions, amount shows linear relationship concentration CP (r = 0.99). Quantitation H. b recovered solubilization with manoxol-1B followed precipitation ethanol also correlates could (66–92% applied CP) salt extraction.