Evaluation of aminolysis of anilinothiazolinones to phenylthiocarbamoyl amino acid methyl amides as an alternative conversion method in protein sequencing

摘要: Abstract The aminolysis of products sequential degradation proteins and peptides by methylamine is an alternative method conversion the unstable 5-alkyl-2-anilino-4-thiazolinones into stable methyl amides N α -phenylthiocarbamoyl amino acids. volatility permits use in gas phase during both manual automatic degradation. Two procedures were studied: (mode A) sequencer reaction chamber after liberation thiazolinones trifluoroacetic acid B) vapors passed through a 1-chlorobutane solution flask sequencer. sequencing program was modified for making standard functions. yields are comparable to those obtained 25% procedure does not affect repetitive yield. Aminolysis on glass filter requires major modification process, yet gives higher degraded derivatives. A disadvantage procedures, especially mode A, presence -methyl- N′ -phenylthiourea amide samples. We have been able achieve expected improvement hydroxy Therefore replacement anilinothiazolinones phenylthiohydantiones routine cannot be recommended. High make promising candidate incorporation fluorescent or other labels