Biochemical Features and Virulence Gene Profiles of Non-O157/O26 Enterohemorrhagic Escherichia coli Strains from Humans in the Yamaguchi Prefecture, Japan

摘要: The biochemical features and virulence gene profiles of 37 enterohemorrhagic Escherichia coli (EHEC) strains belonging to serogroups other than O157 O26 (non-O157/O26 EHEC) were investigated. All isolated from humans between 2002 2013 in the Yamaguchi Prefecture. Serogroup O111 most common, followed by O103, O121, O145. Most (84%) negative for sorbose fermentation, whereas only 1 2 sorbitol rhamnose respectively. Two lacked β-D-glucuronidase activity. Shiga toxin (stx) subtyping revealed 6 genotypes:stx1a (n = 20), stx1a + stx2a 8), 4), stx2b 3), stx2c 1), stx2d 1). Polymerase chain reaction screening adherence genes showed that astA, subA, cdtB present 5, 2, strains, intimin eae was 30 (81%). Of 7 eae-negative saa eibG found 3 respectively; no factors detected remaining strains. antimicrobial susceptibility 12 drugs examined 11 (30%) resistance or more drugs. Our results non-O157/O26 EHEC exhibit various phenotypes carry several toxins factor genes.