Effects of gestational hypertension and pre-eclampsia in mRNA expression of fibrinolysis genes in primary cultured human umbilical vein endothelial cells.

摘要: Hypertension disorders (HD) and pre-eclampsia (PRE) are leading causes of maternal deaths worldwide. PRE is associated with vascular endothelial dysfunction deregulation the fibrinolysis pathway genes. Fibrinolysis fibrin clot hydrolysis process catalyzed by plasmin, a proteolytic enzyme formed from plasminogen. Plasminogen cleaved tissue-type (tPA) urokinase-type (uPA) activators inhibited plasminogen activator inhibitors type-1 (PAI-1) type-2 (PAI-2). The whole maintains blood hemostasis. This study aims to assess PAI-1, PAI-2, tPA uPA mRNA expression in primary cultured human umbilical vein cells (HUVEC) isolated healthy, HD women. Results show that PAI-1 PAI-2 decreased HD-HUVEC, whereas PRE-HUVEC cultures compared control ones. Notably, ratio between pro- anti-fibrinolytic actors remained unchanged among studied groups. It seems newborn's hemostasis maintained balanced probably compensatory mechanism involves changes gene profile. real impact these unknown, however, it suggested could be an increased predisposition disease development progeny.