A simple liposome assay for the screening of zinc ionophore activity of polyphenols.
作者： Gael Clergeaud , Husam Dabbagh-Bazarbachi , Mayreli Ortiz , Juan B. Fernández-Larrea , Ciara K. O’Sullivan
DOI: 10.1016/J.FOODCHEM.2015.11.057
关键词:
摘要: An efficient liposomal system for screening the zinc ionophore activity of a selected library consisting most relevant dietary polyphenols is presented. The was demonstrated by exploring use zinc-specific fluorophore FluoZin-3 loaded liposomes as simple membrane tools that mimic cell membrane. capacity to transport cations across liposome and increase fluorescence encapsulated FluoZin-3. In addition, chelation strength also tested in competition assay based on quenching zinc-dependent emitted zinc-FluoZin-3 complex. Finally, correlation between demonstrated, thus underlining sequestering or ionophoric phenolic compounds can display, thus, providing better knowledge importance structural conformation versus their biological activity. Furthermore, assays developed be used rapid, high-throughput families towards different biometals.
sci-hub.se 参考文章(66)
Robert C. Hider, Zu D. Liu, Hicham H. Khodr, Metal chelation of polyphenols Methods in Enzymology. ,vol. 335, pp. 190- 203 ,(2001) , 10.1016/S0076-6879(01)35243-6
Wei-Qun Ding, Bolin Liu, Joshua L. Vaught, Hanako Yamauchi, Stuart E. Lind, Anticancer Activity of the Antibiotic Clioquinol Cancer Research. ,vol. 65, pp. 3389- 3395 ,(2005) , 10.1158/0008-5472.CAN-04-3577
Detmar Beyersmann, Hajo Haase, Functions of zinc in signaling, proliferation and differentiation of mammalian cells. Biometals. ,vol. 14, pp. 331- 341 ,(2001) , 10.1023/A:1012905406548
Miguel A. ALONSO, Luis CARRASCO, Action of Membrane‐Active Compounds on Mammalian Cells FEBS Journal. ,vol. 109, pp. 535- 540 ,(1980) , 10.1111/J.1432-1033.1980.TB04825.X
D.L. Brautigan, P. Bornstein, B. Gallis, Phosphotyrosyl-protein phosphatase. Specific inhibition by Zn. Journal of Biological Chemistry. ,vol. 256, pp. 6519- 6522 ,(1981) , 10.1016/S0021-9258(19)69016-2
Lars-Oliver Klotz, Klaus-Dietrich Kröncke, Darius P. Buchczyk, Helmut Sies, Role of copper, zinc, selenium and tellurium in the cellular defense against oxidative and nitrosative stress. Journal of Nutrition. ,vol. 133, ,(2003) , 10.1093/JN/133.5.1448S
Hui-Ling Wang, Zheng-Yin Yang, Bao-dui Wang, Synthesis, Characterization and the Antioxidative Activity of Copper(II), Zinc(II) and Nickel(II) Complexes with Naringenin Transition Metal Chemistry. ,vol. 31, pp. 470- 474 ,(2006) , 10.1007/S11243-006-0015-3
Ji-Yong Liang, Yi-Yan Liu, Jing Zou, Renty B. Franklin, Leslie C. Costello, Pei Feng, Inhibitory effect of zinc on human prostatic carcinoma cell growth. The Prostate. ,vol. 40, pp. 200- 207 ,(1999) , 10.1002/(SICI)1097-0045(19990801)40:3<200::AID-PROS8>3.0.CO;2-3
Saul R. Powell, The Antioxidant Properties of Zinc Journal of Nutrition. ,vol. 130, ,(2000) , 10.1093/JN/130.5.1447S
M. Thompson, C.R. Williams, G.E.P. Elliot, Stability of flavonoid complexes of copper(II) and flavonoid antioxidant activity. Analytica Chimica Acta. ,vol. 85, pp. 375- 381 ,(1976) , 10.1016/S0003-2670(01)84703-6