Immobilization of Pepsin onto Chitosan Silica Nanobeads with Glutaraldehyde as Crosslink Agent

作者: Sari Edi Cahyaningrum , Maria Monica Sianita

DOI: 10.9767/BCREC.9.3.7060.263-269

关键词:

摘要: Glutaraldehyde crosslinked chitosan - silica nanobeads have been used as a support in the immobiliza-tion process of pepsin. The effect pH, pepsin concentration, and temperature on characteristics both free immobilized had investigated this study. results showed that im-mobilized has wider pH range, better capacity, performance stability to high temperatures compared with those can be for 8 10 times at which activity remains 20%, whereas only once. These suggested promis-ing applications enzyme biocatalyst. © 2014 BCREC UNDIP. All rights reserved. Received: 1st July 2014; Revised: 3rd September Accepted: How Cite : Cahyaningrum, S.E., Sianita, M.M. (2014). Immobilization Pepsin onto Chitosan Silica Nanobeads Crosslink Agent. Bulletin Chemical Reaction Engineering & Catalysis , 9 (3): 263-269. (doi:10.9767/bcrec.9.3.7060.263-269) Permalink/DOI http://dx.doi.org/10.9767/bcrec.9.3.7060.263-269

参考文章(16)
Gamze Durgun Altun, Senay Akkus Cetinus, Immobilization of pepsin on chitosan beads Food Chemistry. ,vol. 100, pp. 964- 971 ,(2007) , 10.1016/J.FOODCHEM.2005.11.005
C.E. Orrego, N. Salgado, J.S. Valencia, G.I. Giraldo, O.H. Giraldo, C.A. Cardona, Novel chitosan membranes as support for lipases immobilization: Characterization aspects Carbohydrate Polymers. ,vol. 79, pp. 9- 16 ,(2010) , 10.1016/J.CARBPOL.2009.06.015
Jianmin Wu, Mingming Luan, Jiayin Zhao, Trypsin immobilization by direct adsorption on metal ion chelated macroporous chitosan-silica gel beads International Journal of Biological Macromolecules. ,vol. 39, pp. 185- 191 ,(2006) , 10.1016/J.IJBIOMAC.2006.03.017
Elena-Loredana Ticu, Dominique Vercaigne-Marko, Renato Froidevaux, Anca Huma, Vlad Artenie, Didier Guillochon, Use of a protease-modified-alumina complex to design a continuous stirred tank reactor for producing bioactive hydrolysates Process Biochemistry. ,vol. 40, pp. 2841- 2848 ,(2005) , 10.1016/J.PROCBIO.2005.01.003
Linqiu Cao, Luuk van Langen, Roger A Sheldon, Immobilised enzymes: carrier-bound or carrier-free? Current Opinion in Biotechnology. ,vol. 14, pp. 387- 394 ,(2003) , 10.1016/S0958-1669(03)00096-X
Wenshan Liu, Liang Wang, Rongrong Jiang, Specific Enzyme Immobilization Approaches and Their Application with Nanomaterials Topics in Catalysis. ,vol. 55, pp. 1146- 1156 ,(2012) , 10.1007/S11244-012-9893-0
Mona A. Esawy, Yannick Combet-Blanc, Immobilization of Bacillus licheniformis 5A1 milk-clotting enzyme and characterization of its enzyme properties World Journal of Microbiology and Biotechnology. ,vol. 22, pp. 197- 200 ,(2006) , 10.1007/S11274-005-9018-Z
Valentina Nichele, Michela Signoretto, Elena Ghedini, β-Galactosidase entrapment in silica gel matrices for a more effective treatment of lactose intolerance Journal of Molecular Catalysis B-enzymatic. ,vol. 71, pp. 10- 15 ,(2011) , 10.1016/J.MOLCATB.2011.03.002
Jana Frýdlová, Zdenka Kučerová, Marie Tichá, Affinity chromatography of porcine pepsin and pepsinogen using immobilized ligands derived from the specific substrate for this enzyme. Journal of Chromatography B. ,vol. 800, pp. 109- 114 ,(2004) , 10.1016/J.JCHROMB.2003.07.007