Method and test kit for detecting nucleotide variations

摘要: The present invention is related to a method for simultaneous determination of the relative amounts more than one target polynucleotide sequence and nucleotide variations in said targets. carried out by separating recording single-stranded probes, which have hybridized targets are determined distinguished their defined properties including size optional detectable label. probes complementary region that has being contiguous be determined. After with affinity-tagged targets, attached solid support purified. probe hybrids elongated using enzyme-assisted elongations. recorded after release from supports amount each ratio modified sequences calculated results. Also disclosed test kit, kit comprises packaged form devices equipments reagents as well instructions carrying method. useful several diagnostic purposes.