Yeast mutants sensitive to trimethoprim.
作者： JC Game , JG Little , RH Haynes , None
DOI: 10.1016/0027-5107(75)90094-9
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摘要: Abstract Wild-type strains of Saccharomyces cerevisiae are resistant to growth inhibition by the folate antagonist trimethoprim. A mutant strain sensitive trimethoprim was isolated. It found be both ultraviolet light and X-irradiation. Genetic tests revealed that it allelic with a known radiation-sensitive cerevisiae, rad 6-1 . Strains harbouring variety alleles conferring radiation-sensitivity were tested for sensitivity trimetroprim. each four 18 conferred drug, but all other mutants trimethoprim-resistant. All trimethoprim-sensitive strains, including double 6 , gave rise trimethoprim-resistant outgrowths at rather high frequency (∼ 10 −5 ). Several analysed. wide variation in phenotype respect UV-sensitivity found. Genetical analysis resistance resulted from torward mutations separate loci than back or alleles.
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