The Lutropin/Choriogonadotropin Receptor-Induced Phosphorylation of the Extracellular Signal-Regulated Kinases in Leydig Cells Is Mediated by a Protein Kinase A-Dependent Activation of Ras

摘要: The pathways involved in activation of the ERK1/2 cascade Leydig cells were examined MA-10 expressing recombinant human LH receptor (hLHR) and primary cultures rat cell precursors. In hLHR, choriogonadotropin-induced is effectively inhibited by overexpression a cAMP phosphodiesterase (a manipulation that blunts response), addition H89 selective inhibitor protein kinase A), or heat-stable A inhibitor, but not an inactive mutant this inhibitor. Stimulation hLHR did activate Rap1, activated Ras H89-sensitive fashion. Addition to had been cotransfected with guanosine triphosphatase-deficient almost completely hLHR-mediated ERK1/2. We also show 8-bromo-cAMP activates primar...