Molecular profiling of colorectal pulmonary metastases and primary tumours: implications for targeted treatment

摘要: // Sing Y. Moorcraft 1 , Thomas Jones 2 Brian A. Walker George Ladas Eleftheria Kalaitzaki Lina Yuan Ruwaida Begum Zakaria Eltahir Andrew Wotherspoon Angeles Montero-Fernandez Larissa S. Teixeira Mendes David Gonzalez de Castro Sanna Hulkki Wilson Paula Proszek Ye M. To Eliza Hawkes Amitesh Roy Cunningham Sheela Rao Watkins Naureen Starling Anne Bowcock 3 and Ian Chau The Royal Marsden NHS Foundation Trust, London Sutton, United Kingdom Brompton Harefield London, National Heart Lung Institute, Imperial College, Correspondence to: Chau, email: ian.chau@rmh.nhs.uk Keywords: colorectal cancer, heterogeneity, metastasectomy, pulmonary metastases, RAS Received: October 06, 2016      Accepted: March 29, 2017      Published: April 11, 2017 ABSTRACT This study aimed to molecularly characterise metastases (PM) investigate whether their molecular profiles were concordant with those of the primary tumour. Clinical data archival formalin fixed paraffin embedded tissue samples retrospectively collected from patients who underwent ≥ metastasectomies for cancer between 1997–2012. Primary tumour metastatic analysed using a targeted capture sequencing panel 46 cancer-associated genes. 5-year progression-free overall survival rates 81 in this 32% (95% CI 22–42%) 77% 66–85%) respectively. Fifty-four had available PM, successfully obtained 33 PM 24 patients. most frequently mutated genes APC (71%), KRAS (58%) TP53 (46%). Seventy-three percent 15 matched 6 7 (86%) profiles. concordance NRAS was 100%. At our institutions, resectable favourable prognosis. mutations commonly detected highly