Preparation of a TiO2 nanoparticle-deposited capillary column by liquid phase deposition and its application in phosphopeptide analysis.
作者： Bo Lin , Ting Li , Yong Zhao , Fang-Ke Huang , Lin Guo
DOI: 10.1016/J.CHROMA.2008.03.043
关键词:
摘要: Analysis of phosphopeptides from complex mixtures derived proteolytic digestion biological samples is a challenging yet highly important task. Since are usually present in small amounts, enrichment often necessary prior to their characterization by mass spectrometry. In this study, thin layer titanium dioxide (TiO2) nanoparticles (NPs) was deposited onto the surface capillary column liquid phase deposition (LPD) technique and applied selectively concentrate protein digest products. This is, our knowledge, first demonstration using construct in-tube solid microextraction devices for analysis. By coupling device off-line or on-line with spectrometry analysis, experiments systematic optimization loading washing conditions were carried out, good trapping selectivity TiO2 NP-deposited columns towards demonstrated.
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sci-hub.se 参考文章(46)
S DEKI, Y AOI, A KAJINAMI, A novel wet process for the preparation of vanadium dioxide thin film Journal of Materials Science. ,vol. 32, pp. 4269- 4273 ,(1997) , 10.1023/A:1018603402586
Huilin Zhou, Julian D. Watts, Ruedi Aebersold, A systematic approach to the analysis of protein phosphorylation Nature Biotechnology. ,vol. 19, pp. 375- 378 ,(2001) , 10.1038/86777
R. Honkanen, T. Golden, Regulators of serine/threonine protein phosphatases at the dawn of a clinical era? Current Medicinal Chemistry. ,vol. 9, pp. 2055- 2075 ,(2002) , 10.2174/0929867023368836
Lin Guo, Carl J. Kozlosky, Lowell H. Ericsson, Thomas O. Daniel, Douglas P. Cerretti, Richard S. Johnson, Studies of ligand-induced site-specific phosphorylation of epidermal growth factor receptor. Journal of the American Society for Mass Spectrometry. ,vol. 14, pp. 1022- 1031 ,(2003) , 10.1016/S1044-0305(03)00206-X
Akira SANO, Hiroshi NAKAMURA, Titania as a chemo-affinity support for the column-switching HPLC analysis of phosphopeptides: application to the characterization of phosphorylation sites in proteins by combination with protease digestion and electrospray ionization mass spectrometry. Analytical Sciences. ,vol. 20, pp. 861- 864 ,(2004) , 10.2116/ANALSCI.20.861
Shigehito Deki, Yoshifumi Aoi, Osamu Hiroi, Akihiko Kajinami, Titanium (IV) Oxide Thin Films Prepared from Aqueous Solution Chemistry Letters. ,vol. 25, pp. 433- 434 ,(1996) , 10.1246/CL.1996.433
Derek T. McLachlin, Brian T. Chait, Improved β-Elimination-Based Affinity Purification Strategy for Enrichment of Phosphopeptides Analytical Chemistry. ,vol. 75, pp. 6826- 6836 ,(2003) , 10.1021/AC034989U
David N. Perkins, Darryl J. C. Pappin, David M. Creasy, John S. Cottrell, Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis. ,vol. 20, pp. 3551- 3567 ,(1999) , 10.1002/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2
S. Deki, Y. Aoi, Y. Miyake, A. Gotoh, A. Kajinami, Novel wet process for preparation of vanadium oxide thin film Materials Research Bulletin. ,vol. 31, pp. 1399- 1406 ,(1996) , 10.1016/0025-5408(96)00136-5
Greg T. Cantin, Teresa R. Shock, Sung Kyu Park, Hiten D. Madhani, John R. Yates, Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry Analytical Chemistry. ,vol. 79, pp. 4666- 4673 ,(2007) , 10.1021/AC0618730