Synaptic plasticity processes underlying consolidation and reconsolidation of Pavlovian conditioning

摘要: In the field of drug addiction, relapse back to seeking and taking is major unmet clinical need. The rate drug-taking ~70-80% within a year abstinence. Gaining better understanding prolonged neuronal changes that have taken place during addiction may lead design anti-relapse therapies. It now widely believed one component by aberrant learning memory processes. To study this, we investigated synaptic caused development drug-seeking behaviour in C57BL/6J mice. Mice were treated either with non-contingent morphine or trained exhibit following morphine-induced conditioned preference (CPP) training, hippocampal slices from these animals examined at CA3-CA1 synapse using electrophysiological methods. underwent CPP demonstrated significant for paired compartment before ex vivo analysis. Using recordings, both treatments resulted reduced ability undergo stimulus-induced LTP compared their respective controls. Whole cell patch clamp was then utilised further investigate effects. Non-contingent treatment pre- post-synaptic an increased AMPA:NMDA receptor ratio, concurrent increases size, reductions release probability glutamate GABA. Morphine more variable increase ratio (presumably same mechanism but specific group neurones) GABA also decreased. There no detected size however, any probability. These findings therefore reveal set adaptations hippocampus unique behavioural change, provide targets future intervention addiction.