摘要: EVIDENCE for the earlier suggested1 participation of a flavin compound in pyruvic acid oxidation by Bacterium Delbruckii has been obtained procedure similar to that used Warburg and Christian2 remove prosthetic group (flavin-adenin dinucleotide) from amino-acid oxidase. Phosphate extracts derived lactic bacteria were repeatedly precipitated with 50 per cent ammonium sulphate at pH. 3 1–2° C. By this treatment protein fraction was obtained, which on addition thiamin (vitamin B1) pyrophosphate only1, did not catalyse oxidation, but so together flavin-adenin dinucleotide, kindly supplied me Prof. O. Warburg.
harvard.edu
cabdirect.org
nature.com
sci-hub.se 参考文章(4)
F. B. STRAUB, H. S. CORRAN, D. E. GREEN, Mechanism of the Oxidation of Reduced Coensyme I Nature. ,vol. 143, pp. 119- 119 ,(1939) , 10.1038/143119A0
R. KIRK, Sulphanilamides and Rabies Nature. ,vol. 143, pp. 77- 77 ,(1939) , 10.1038/143077A0
Fritz Lipmann, Gerty Perlmann, Hydrogenation of Vitamin B1 and Other Quaternary Thiazoles1 Journal of the American Chemical Society. ,vol. 60, pp. 2574- 2578 ,(1938) , 10.1021/JA01278A005
John George Dewan, David Ezra Green, Coenzyme factor—a new oxidation catalyst1 Biochemical Journal. ,vol. 32, pp. 626- 639 ,(1938) , 10.1042/BJ0320626