MutY catalytic core, mutant and bound adenine structures define specificity for DNA repair enzyme superfamily
作者： Yue Guan , Raymond C. Manuel , Andrew S. Arvai , Sudip S. Parikh , Clifford D. Mol
DOI: 10.1038/4168
关键词:
摘要: The DNA glycosylase MutY, which is a member of the Helix-hairpin-Helix (HhH) superfamily, excises adenine from mispairs with 8-oxoguanine and guanine. High-resolution crystal structures MutY catalytic core (cMutY), complex bound adenine, designed mutants reveal basis for specificity glycosyl bond cleavage chemistry. two cMutY helical domains form positively-charged groove adenine-specific pocket at their interface. Watson-Crick hydrogen partners are substituted by protein atoms, confirming nucleotide flipping mechanism, supporting specific binding orientation structurally related glycosylases.
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