A rapid and sensitive screening kit for the detection of anti-Toxocara larval ES antibodies

作者: Nobuaki Akao , Albert E. Chu , Setsuko Tsukidate , Koichiro Fujita

DOI: 10.1016/S1383-5769(97)00027-5

关键词:

摘要: Abstract We developed a new screening kit for the detection of anti-Toxocara larval excretory-secretory antibodies. The test can be performed within 3 min one sample and does not require high-priced supplemental instrument. Moreover, results are easily directly observed. Using this kit, 22 sera taken from healthy subjects were negative antibodies at serum dilution 1:20. Of 14 proven cases parasitic infections other than toxocariasis, (gnathostomiasis) showed positive result, but others negative. In serologically diagnosed good correlation with ELISA, immunoblot double gel diffusion tests. designated as ToxocaraCHEK.

参考文章(22)
Khattab Hm, Azab Me, Bebars Ma, Safar Eh, Khalil Hm, el Hady H, Immunodiagnostics of Toxocara canis in suspected ocular and visceral manifestations. Folia Parasitologica. ,vol. 37, pp. 249- 254 ,(1990)
Lawrence T. Glickman, Jean-Francois Magnaval, Zoonotic roundworm infections. Infectious Disease Clinics of North America. ,vol. 7, pp. 717- 732 ,(1993) , 10.1016/S0891-5520(20)30551-1
Yves Carlier, D Bout, André Capron, J. Yang, The use of an excretory-secretory antigen for an ELISA specific sero-diagnosis of visceral larva migrans. Biomedicine & Pharmacotherapy. ,vol. 36, pp. 39- 42 ,(1982)
P Jacquier, B Gottstein, Y Stingelin, J Eckert, Immunodiagnosis of toxocarosis in humans: evaluation of a new enzyme-linked immunosorbent assay kit. Journal of Clinical Microbiology. ,vol. 29, pp. 1831- 1835 ,(1991) , 10.1128/JCM.29.9.1831-1835.1991
J F Magnaval, R Fabre, P Maurières, J P Charlet, B de Larrard, Evaluation of an immunoenzymatic assay detecting specific anti-Toxocara immunoglobulin E for diagnosis and posttreatment follow-up of human toxocariasis. Journal of Clinical Microbiology. ,vol. 30, pp. 2269- 2274 ,(1992) , 10.1128/JCM.30.9.2269-2274.1992
Neil R. Lynch, Kim Eddy, A.Neill Hodgen, Reina I. Lopez, Keven J. Turner, Seroprevalence of Toxocara canis infection in tropical Venezuela Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 82, pp. 275- 281 ,(1988) , 10.1016/0035-9203(88)90446-4
B. Gueglio, L. de Gentile, J. M. Nguyen, J. Achard, D. Chabasse, M. Marjolet, Epidemiologic approach to human toxocariasis in western France Parasitology Research. ,vol. 80, pp. 531- 536 ,(1994) , 10.1007/BF00932703