RNA-Mediated Reprogramming of Primary Adult Human Dermal Fibroblasts into c-kit+ Cardiac Progenitor Cells

摘要: Cardiovascular disease is the leading cause of death in United States. Heart failure a common, costly, and potentially fatal condition that inadequately managed by pharmaceuticals. Cardiac repair therapies are promising alternative options. A potential cardiac therapy involves reprogramming human fibroblasts toward an induced progenitor-like state. We developed clinically useful safer method nonintegrative delivery cocktail transcription factor-encoding mRNAs into autologous dermal obtained from skin biopsies. Using this method, adult neonatal were reprogrammed progenitor cells (CPCs) expressed c-kit, Isl-1, Nkx2.5. Furthermore, these CPCs differentiated cardiomyocytes (CMs) vitro as judged increased expression troponin T, α-sarcomeric actinin, RyR2, SERCA2 displayed enhanced caffeine-sensitive calcium release. The ability to reprogram pa...