COMMUNAUTE FRANCAISE DE BELGIQUE ACADEMIE UNIVERSITAIRE WALLONIEEUROPE UNIVERSITE DE LIEGE GEMBLOUX AGROBIO TECH Identification of Pythium species inducing common bean ( Phaseolus vulgaris L.) root rot symptoms and development of backcrosses to improve the level of varietal resistance to this disease

摘要: 29 INTRODUCTION 31 MATERIALS AND METHODS 33 Collection of samples and purification the inducing Pythium agents DNA extraction 34 Polymerase Chain Reaction Sequencing amplified identification 35 Pathogenicity analysis species 36 RESULTS 37 Sample collection characterization isolated 42 DISCUSSION 49 REFERENCES 54 CHAPTER IV 58 INTROGRESSION OF PYTHIUM ROOT ROT RESISTANCE GENE INTO RWANDAN SUSCEPTIBLE COMMON BEAN CULTIVARS Abstract 59 Materials Methods 64 Genetic materials study site Planting conditions 65 Hybridization protocol 66 Leaf preparation 67 analyses 68